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KMID : 0380019950100050491
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 5 p.491 ~ p.497
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Formation of Cyclodextrin Adsorbent Using fatty Acid as a Ligand and Fractionation of α-, β- and γ-Cyclodextrins
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Abstract
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In order to fractionate a-, 6- and y-cyclodextrins(CDs) from CD reaction mixture, various CD adsorbents were manufactured using fatty acids as the ligand molecules and anion exchange resins as matrix. Among several anion exchange resins, DEAE Cellulose was found to be the most suitable matrix for binding fatty acid. The binding stability between DEAE Cellulose and capric acid was tested under the various operation conditions, such as temperature, ethanol concentration, and ionic strength. Specific CD adsorbents manufactured with different chain-length fatty acids, saturated and unsaturated, were compared in terms of the recovery yield and selectivity of a-, 8- and y-CDs. Stearic acid(C,,, saturated) was identified as the most effective ligand for fractionation of a-CD, and linoleic acid(C,b, unsaturated) for j9-CD. The spacer length between the matrix and ligand was required for effective adsorption of CDs, and the double bond in fatty acid molecules was also acted as an important factor determining recovery yield and selectivity. The elution patterns of a- and BCD from column packed with stearic acid and linoleic acid CD adsorbents were also investigated at the various elution conditions for fractionation of a- and /9-CD.
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